Part:BBa_K1432001:Experience

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Applications of BBa_K1432001

We will construct a mlr promoter-GPF-mlrA cluster and clone it into the E. coli-L. lactis shuttle expression vector. So it can be easily constructed in the laboratory and could avoid secondary pollution in the natural environment of E.coli.

we synthesized mlrA gene, there are 336 codons in Sphingomonas species ACM-3962. We replace 246 of them in order to increase the expression of mlrA because they are not abundant codons in Lactococcus lactis. And we successfully did it both in E.coli and L.lactis. When we induced the expression of protein, it shows that L.lactis could be more efficiently expressed than E.coli.

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